Resistance of potato clones to necrotic recombinant strains of potato virus y (pvy)

The Ry adg allele is widely used by breeders to confer extreme resistance to all strains of PVY. However, the necrotic strain has increased recombination resulting in recent considerable losses in productivity. Thus far, not all necrotic recombinant strains of PVY have been tested for their reaction...

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Veröffentlicht in:Ciência e agrotecnologia 2014-08, Vol.38 (4), p.343-351
Hauptverfasser: Ribeiro, Silvia Regina Rodrigues de Paula, Pinto, César Augusto Brasil Pereira, Costa, Suellen Bárbara Ferreira Galvino, Menezes, Mariney de, Figueira, Antonia dos Reis
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Sprache:eng
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Zusammenfassung:The Ry adg allele is widely used by breeders to confer extreme resistance to all strains of PVY. However, the necrotic strain has increased recombination resulting in recent considerable losses in productivity. Thus far, not all necrotic recombinant strains of PVY have been tested for their reaction to the Ry gene. The objective of this study was to identify potato clones carrying the resistant allele and to assess their reaction to the following recombinant strains: NTN (PVY NTN), Wilga (PVY N-Wi), and "curly top" (PVY E). Advanced clones from the potato breeding program at Universidade Federal de Lavras were evaluated through a specific molecular marker for the Ry adg allele. The clones carrying the resistance allele were grafted on tobacco plants infected with necrotic recombinant strains of PVY. The clones carrying the allele for resistance to PVY were not infected with any of the recombinants during the grafting test. These results confirm that resistance to necrotic recombinant strains has not yet been overcome and that the Ry adg allele also confers resistance to the three recombinant strains tested. O alelo Ry adg é muito utilizado pelos melhoristas, por conferir resistência extrema a todas as estirpes do PVY. Entretanto, a estirpe necrótica possui grande capacidade de recombinação, de modo que, novos variantes genéticos capazes de provocar perdas consideráveis na produtividade, vêm surgindo recentemente. Até o momento, nem todas as estirpes recombinantes de PVY necrótico foram testados quanto à sua reação com o gene Ry. Neste estudo, objetivou-se identificar clones de batata, portadores do alelo de resistência e avaliar a sua reação aos seguintes isolados recombinantes: NTN (PVY NTN), Wilga (PVY N-Wi) e "top crespo" (PVY E). Clones avançados de batata, do programa de melhoramento da Universidade Federal de Lavras, foram avaliados por meio de um marcador molecular específico para o alelo Ry adg. Os clones portadores do alelo de resistência foram enxertados em plantas de fumo infectadas com o PVY N e três isolados recombinantes. Os clones portadores do alelo de resistência não foram infectados com nenhum dos recombinantes e nem com o PVY N no teste de enxertia. Esses resultados confirmam que o alelo Ry adg confere resistência também aos variantes testados.
ISSN:1413-7054
1981-1829
1413-7054
DOI:10.1590/S1413-70542014000400004