CADEE : Computer-Aided Directed Evolution of Enzymes

The tremendous interest in enzymes as biocatalysts has led to extensive work in enzyme engineering, as well as associated methodology development. Here, a new framework for computer-aided directed evolution of enzymes ( ) is presented which allows a drastic reduction in the time necessary to prepare and analyze semi-automated directed evolution of enzymes. A pedagogical example of the application of to a real biological system is also presented in order to illustrate the workflow.