Probiotic Properties and In vitro Biosafety Assessment of Human Breast Milk Isolates

Human milk can be an important source for obtaining potential probiotics strains for newborns in order to establish the beneficial gut microbial community and development of immune system. The aim of the study was to explore potential human breast milk probiotics and to carry out their in vitro biosafety assessment. The study obtained three isolates namely, [SP.sub.1]B, [B.sub.2]Enr and [SP.sub.1] which showed potential probiotic activities compared to standard probiotic Lactobacillusplantarum. In addition, these isolates were found to be safe through various in vitro biosafety aspects. The molecular identification by 16srDNA sequencing revealed that [SP.sub.1]B and [B.sub.2]Enr belong to Bacillus cereus (MK210172) and Staphylococcus epidermidis (MK210234), respectively. For the first time, the study suggests that these bacterial strains may come in the category of probiotics and can be considered further after in vivo biosafety assessments. Keywords: Probiotics; Biosafety assessment; Human breast milk; 16srDNA sequencing.