Analysis of insoluble proteins

The analysis of insoluble proteins represents a major technical challenge for the field of proteomics. For example, membrane proteins are often insoluble in common solvents and represent 20-30% of the proteins encoded by the human genome. Chemical analysis on an individual basis is often required an...

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Veröffentlicht in:	BioTechniques 2009-05, Vol.46 (6), p.409-419
Hauptverfasser:	Trimpin, Sarah, Brizzard, Bill
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals fusion tag Humans IMS MALDI Mass Spectrometry Proteins - analysis Proteins - chemistry Proteomics recombinant protein Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism Solubility solvent-free mass spectrometry Solvents
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Beschreibung
Zusammenfassung:	The analysis of insoluble proteins represents a major technical challenge for the field of proteomics. For example, membrane proteins are often insoluble in common solvents and represent 20-30% of the proteins encoded by the human genome. Chemical analysis on an individual basis is often required and is laborious and time-consuming. This review presents an overview of methods for purification of expressed proteins using fusion tags as well as methods for analysis of insoluble proteins by mass spectrometry with a goal of achieving high-throughput analysis.
ISSN:	0736-6205 1940-9818
DOI:	10.2144/000113168