Serine Palmitoyltransferase, the First Step Enzyme in Sphingolipid Biosynthesis, Is Involved in Nonhost Resistance

An overexpression screen of Nicotiana benthamiana cDNAs identified a gene for the LCB2 subunit of serine palmitoyltransferase (SPT) as a potent inducer of hypersensitive response-like cell death. The pyridoxal 5'-phosphate binding site of NbLCB2 is required for its function as a cell death indu...

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Veröffentlicht in:	Molecular plant-microbe interactions 2009, Vol.22 (1), p.31-38
Hauptverfasser:	TAKAHASHI, Yoshihiro, BERBERICH, Thomas, KANZAKI, Hiroyuki, MATSUMURA, Hideo, SAITOH, Hiromasa, KUSANO, Tomonobu, TERAUCHI, Ryohei
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Biological and medical sciences Fatty Acids, Monounsaturated - pharmacology Fundamental and applied biological sciences. Psychology Immunity, Innate - drug effects Immunity, Innate - genetics Immunoblotting Models, Biological Molecular Sequence Data Nicotiana - enzymology Nicotiana - metabolism Nicotiana - microbiology Nicotiana benthamiana Phytopathology. Animal pests. Plant and forest protection Plant Diseases - genetics Plant Diseases - microbiology Plant Leaves - enzymology Plant Leaves - metabolism Plant Leaves - microbiology Plant Proteins - antagonists & inhibitors Plant Proteins - genetics Plant Proteins - metabolism Pseudomonas - growth & development Pseudomonas cichorii Reverse Transcriptase Polymerase Chain Reaction Sequence Homology, Amino Acid Serine C-Palmitoyltransferase - antagonists & inhibitors Serine C-Palmitoyltransferase - genetics Serine C-Palmitoyltransferase - metabolism Sphingolipids - biosynthesis
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Zusammenfassung:	An overexpression screen of Nicotiana benthamiana cDNAs identified a gene for the LCB2 subunit of serine palmitoyltransferase (SPT) as a potent inducer of hypersensitive response-like cell death. The pyridoxal 5'-phosphate binding site of NbLCB2 is required for its function as a cell death inducer. NbLCB2 mRNA is accumulated after infection by nonhost pathogen Pseudomonas cichorii. Resistance of N. benthamiana against P. cichorii was compromised by treatment with an SPT inhibitor and in NbLCB2- and NbLCB1-silenced plants. These results suggest that biosynthesis of sphingolipids is necessary for the nonhost resistance of N. benthamiana against P. cichorii.
ISSN:	0894-0282 1943-7706
DOI:	10.1094/mpmi-22-1-0031