Gene Replacement in Gram-Negative Bacteria: the pMAKSAC Vectors

Gene Replacement in Gram-Negative Bacteria: the pMAKSAC Vectors

The implementation of targeted modifications in genomes through homologous recombination has become an important and widely used genetic tool. In the case of bacteria, a number of strategies introduce short deletions and insertions, or even entire foreign genes or operons in the chromosome of a give...

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Veröffentlicht in:BioTechniques 2000-02, Vol.28 (2), p.198-204
Hauptverfasser: Favre, Didier, Viret, Jean-François
Format: Artikel
Sprache:eng
Schlagworte:
Bacillus subtilis - genetics
Biological and medical sciences
Biotechnology
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Genetic engineering
Genetic technics
Genetic Techniques
Genetic Vectors
Gram-Negative Bacteria - genetics
Hexosyltransferases - genetics
Methods. Procedures. Technologies
plasmid pMAKSAC
Plasmids - genetics
Restriction Mapping
Vectors (cloning, transfer, expression). Insertion sequences and transposons
Vibrio cholerae - genetics
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Zusammenfassung:The implementation of targeted modifications in genomes through homologous recombination has become an important and widely used genetic tool. In the case of bacteria, a number of strategies introduce short deletions and insertions, or even entire foreign genes or operons in the chromosome of a given host.
ISSN:0736-6205
1940-9818
DOI:10.2144/00282bm02