Larvicidal and Enzymatic Inhibition Effects of Annona muricata Seed Extract and Main Constituent Annonacin against Aedes aegypti and Aedes albopictus (Diptera: Culicidae)

The mosquitoes and are vectors of arboviruses that cause dengue, zika and chikungunya. Bioactive compounds from plants are environmentally sustainable alternatives to control these vectors and thus the arboviruses transmitted by them. The present study evaluated the larvicidal activity of an acetogenin-rich fraction (ACERF) and its main constituent annonacin obtained from seeds on and . The larvicidal assays were performed using different concentrations to calculate the LC and LC values observed 24 h after exposure to the treatment. Annonacin was more active against (LC 2.65 μg·mL ) in comparison with (LC 8.34 μg·mL ). In contrast, the acetogenin-rich fraction was more active against (LC 3.41 μg·mL ) than (LC 12.41 μg·mL ). ACERF and annonacin treated larvae of and showed significant differences in the inhibition of their metabolic enzymes when compared to untreated larvae. The results demonstrate the relevant larvicidal action of the acetogenin-rich fraction and annonacin showing the potential to develop new products for the control of and .