Quantification of DNA through the NanoDrop Spectrophotometer: Methodological Validation Using Standard Reference Material and Sprague Dawley Rat and Human DNA

Quantification of DNA through the NanoDrop Spectrophotometer: Methodological Validation Using Standard Reference Material and Sprague Dawley Rat and Human DNA

This study aimed to validate an analytical method to determine DNA concentration using standard reference material (NIST SRM 2372) and Sprague Dawley rat and human DNA. Microvolumes were used to analyse DNA samples. Linearity showed correlation coefficients higher than R ≥ 0.9950, and the precision...

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Veröffentlicht in:International journal of analytical chemistry 2020, Vol.2020 (2020), p.1-9
Hauptverfasser: Rascón-Durán, María Lucila, Guadalupe Corella-Madueño, María Alba, Pérez-Martínez, Cinthia Jhovanna, Anduro-Corona, Iván, García-Alegría, Alejandro Monserrat, Astiazaran-Garcia, Humberto
Format: Artikel
Sprache:eng
Schlagworte:
Analytical chemistry
Bias
Calibration
Confidence intervals
Correlation analysis
Correlation coefficients
Deoxyribonucleic acid
DNA
DNA methylation
Laboratories
Linearity
Molecular biology
Optical instruments
Recovery
Reference materials
Standard deviation
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Zusammenfassung:This study aimed to validate an analytical method to determine DNA concentration using standard reference material (NIST SRM 2372) and Sprague Dawley rat and human DNA. Microvolumes were used to analyse DNA samples. Linearity showed correlation coefficients higher than R ≥ 0.9950, and the precision value was ≤2% CV. Trueness based on bias and the percentage of recovery showed bias values lower than Z-test with a 95% confidence level and a recovery percentage within the range (% Rec = 100% ± 5%), and the stability of the samples was 60 days (2–4°C).
ISSN:1687-8760
1687-8779
DOI:10.1155/2020/8896738