Editing of a Novel Cd Uptake-Related Gene CUP1 Contributes to Reducing Cd Accumulations in Arabidopsis thaliana and Brassica napus

is a Cd hyperaccumulator, which is a serious threat to food and fodder safety. However, no related studies on developing Cd-safe have been reported yet. Here, we screened out a novel Cd uptake-related gene, from the major facilitator superfamily in . The mutation of decreased Cd accumulation, both in roots and shoots of . Furthermore, the disruption of the gene by the CRISPR/Cas9 system significantly reduced Cd accumulation in . Interestingly, the disruption of the gene, an orthologous gene of , by the CRISPR/Cas9 system also diminished Cd accumulation in both roots and shoots of based on the hydroponics assay. Furthermore, for the field experiment, the Cd accumulations of -edited lines were reduced by 52% in roots and 77% in shoots compared to that of wild-type (WT) lines, and the biomass and yield of -edited lines increased by 42% and 47% of that of WT, respectively. Noteworthily, agronomic characteristics of were not apparently affected by -editing. Thus, -edited lines are excellent non-transgenic germplasm resources for reducing Cd accumulation without a distinct compromise in yield, which could be applied to agricultural production in Cd-contaminated soils.