Effects of Probiotic Compound Preparations on Antioxidant Indexes, Cytokines and Intestinal Flora in Mice Treated with Ceftriaxone Sodium

Objective: To explore the effect of a probiotic compound preparation composed of Bifidobacterium longum BB536 and Bifidobacterium lactis HN019 on dysbiosis of murine gut microbiota induced by ceftriaxone sodium. Methods: Ceftriaxone sodium (2 mg/g) was given to mice for 5 days to construct intestinal flora dysbiosis model mice and then they were randomly divided into model group, low-dose (2×105 CFU/g), medium-dose (4×105 CFU/g) and high-dose (1.2×106 CFU/g) groups of probiotic compound preparations. In addition, normal mice were set as the control group. From the 6th day, each dose group was administered with the corresponding dose of probiotic compound preparation, the control group and model group were administered with equal volume of normal saline for 30 days. After the gavage, the mice feces were collected to count the intestinal flora and 16S rDNA high-throughput sequencing was performed to analyze the diversity and structure of the flora. The levels of IL-2, IL-6, IL-1β and TNF-α in serum were measure