Cyclopropane-Containing Fatty Acids from the Marine Bacterium Labrenzia sp. 011 with Antimicrobial and GPR84 Activity

Bacteria of the family Rhodobacteraceae are widespread in marine environments and known to colonize surfaces, such as those of e.g., oysters and shells. The marine bacterium sp. 011 is here investigated and it was found to produce two cyclopropane-containing medium-chain fatty acids ( , ), which inhibit the growth of a range of bacteria and fungi, most effectively that of a causative agent of Roseovarius oyster disease (ROD), DSM 16950. Additionally, compound acts as a potent partial, β-arrestin-biased agonist at the medium-chain fatty acid-activated orphan G-protein coupled receptor GPR84, which is highly expressed on immune cells. The genome of sp. 011 was sequenced and bioinformatically compared with those of other spp. This analysis revealed several cyclopropane fatty acid synthases (CFAS) conserved in all strains analyzed and a putative gene cluster encoding for two distinct CFASs is proposed as the biosynthetic origin of and .