Protocol for laser-induced chronic ocular hypertension and intracameral injection in nonhuman primates
Laser-induced hypertension in nonhuman primates is used to mimic human glaucoma, the leading cause of irreversible blindness. In this protocol, we detail steps for laser-induced ocular hypertension in nonhuman primates by laser photocoagulation of the trabecular meshwork and subsequent intracameral...
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Veröffentlicht in: | STAR protocols 2022-12, Vol.3 (4), p.101801-101801, Article 101801 |
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Sprache: | eng |
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Zusammenfassung: | Laser-induced hypertension in nonhuman primates is used to mimic human glaucoma, the leading cause of irreversible blindness. In this protocol, we detail steps for laser-induced ocular hypertension in nonhuman primates by laser photocoagulation of the trabecular meshwork and subsequent intracameral injection. We further describe recording and evaluation of intraocular pressure changes and peripapillary retinal nerve fiber layer thickness. This protocol can assist researchers improve the success rate and repeatability of the procedure and reduce the number of nonhuman primates needed.
For complete details on the use and execution of this protocol, please refer to Sun et al. (2022).
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•Detailed description for general and ophthalmological examination before experiment•Protocol to generate laser-induced chronic ocular hypertension in rhesus monkeys•Laser photocoagulation of the trabecular meshwork followed by intracameral injection•Approach is highly reproducible and offers a tool to test potential treatment strategies
Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
Laser-induced hypertension in nonhuman primates is used to mimic human glaucoma, the leading cause of irreversible blindness. In this protocol, we detail steps for laser-induced ocular hypertension in nonhuman primates by laser photocoagulation of the trabecular meshwork and subsequent intracameral injection. We further describe recording and evaluation of intraocular pressure changes and peripapillary retinal nerve fiber layer thickness. This protocol can assist researchers improve the success rate and repeatability of the procedure and reduce the number of nonhuman primates needed. |
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ISSN: | 2666-1667 2666-1667 |
DOI: | 10.1016/j.xpro.2022.101801 |