The isolation of (6S, 9S)-cyclo (prolylvalyl) from marine actinomycete, by use of high speed contercurrent chromatography

The marine actinomycete B 1758 came from the actinomycete collection of the Alfred Wegener Institute for Polar and Marine Research in Bremerhafen Germany. 1.079 g of raw extract was obtained by fermentation. Seven fractions were separated by column chromatography on silica gel. Fraction 4 was separated by high speed countercurrent chromatography. Fraction 4.6 yielded 5.2 mg of (6S, 9S)-cyclo(prolylvalyl). Morska aktinomiceta B 1758 potice iz zbirke aktinomiceta instituta Alfred Wegener za polarna i morska istrazivanja u Bremerhafen-u, Nemacka. Fermentacijom je dobijeno 1,079 g sirovog ekstrakta. Kolonskom hromatografijom na silikagelu je razdvojeno sedam frakcija. Cetvrta frakcija je razdvojena koriscenjem HSCCC hromatografije. U frakciji 4.6 je dobijeno 5,2 mg (6S, 9S)-ciklo (prolilvalila).