Evaluating RHAU140-CFP molecule for specific recognition of parallel DNA G-Quadruplex by the fluorescence spectrophotometer

Determination of G-quadruplex formation in DNA is required for the design of small molecules (drugs) targeting G-quadruplex. In previous studies, we used the fluorescent protein RHAU53-CFP capable of recognizing parallel G-quadruplex structures through a fluorescent signal. Herein, we evaluated the ability to specifically recognize the parallel G-quadruplex DNA structure of RHAU140-CFP by fluorescence spectrophotometer. The results showed that the fluorescent protein probe was able to recognize the parallel G-quadruplex structure. In addition, the fluorescence signal showed that the sensitivity of the RHAU140-CFP protein was three times higher than that of the RHAU53-CFP fluorescent signal in determining the formation of a G-quadruplex structure. Fluorescence protein probe sensitivity would provide a potential tool for the detection of parallel G-quadruplex both in vitro and in vivo.