Evaluation of Microleakage of Orthograde Root-Filling Materials in Immature Permanent Teeth

Introduction: The absence of a barrier in an open root apex makes endodontic treatment challenging as root-filling material can easily reach the surrounding tissue. The aim of the study was to compare the apical microleakage associated with mineral trioxide aggregate (MTA), biodentine, custom-made gutta-percha with MTA plus and custom-made gutta-percha with Adseal in immature permanent teeth by dye penetration method. Methods: Apical 2mm of 60 single-rooted mandibular premolar teeth was resected to create divergent open apices and 10 teeth each were filled with Biodentine plug, MTA plug, custom-made gutta-percha with MTA plus sealer and custom-made gutta-percha with Adseal sealer. Ten teeth each acted as positive and negative controls. All the samples were stored at 37°C at 100% humidity for 5weeks and then immersed in 2% Rhodamine B dye for 24h. Transverse sectioning was done apically at 1mm and 3mm to evaluate dye penetration under a fluorescence microscope using ImageJ software. Results: There was a significantly greater microleakage at 1mm cross section compared to 3mm (p