Evaluation of an RBD-nucleocapsid fusion protein as a booster candidate for COVID-19 vaccine

Despite successful vaccines and updates, constant mutations of SARS-CoV-2 makes necessary the search for new vaccines. We generated a chimeric protein that comprises the receptor-binding domain from spike and the nucleocapsid antigens (SpiN) from SARS-CoV-2. Once SpiN elicits a protective immune response in rodents, here we show that convalescent and previously vaccinated individuals respond to SpiN. CD4+ and CD8+ T cells from these individuals produced greater amounts of IFN−γ when stimulated with SpiN, compared to SARS-CoV-2 antigens. Also, B cells from these individuals were able to secrete antibodies that recognize SpiN. When administered as a boost dose in mice previously immunized with CoronaVac, ChAdOx1-S or BNT162b2, SpiN was able to induce a greater or equivalent immune response to homologous prime/boost. Our data reveal the ability of SpiN to induce cellular and humoral responses in vaccinated human donors, rendering it a promising candidate. [Display omitted] •SpiN induced IFN-Υ in CD4 and CD8 T cells of convalescent and vaccinated individuals•IgG from convalescent and vaccinated individuals recognized SpiN•Mice respond similarly or better when boosted with SpiN than with commercial vaccines Health sciences; Public health; Immunology; Virology