Fabrication of green nanoinsecticides from agri-waste of corn silk and its larvicidal and antibiofilm properties

The corn silk (CS) is composed of the thread-like stigmas of female inflorescences of L. and represents an important waste material from maize crop production that can be recycled in further applications. In this research, the CS was used for the bio-fabrication of Ag nanoparticles (AgNPs) that were evaluated against (I–V) larval instars and pupae of the mosquito vector . CS-AgNPs were characterized by UV-Vis spectroscopy, TEM, EDAX, XRD, FTIR, DLS, and zeta potential analysis. extract analyzed by gas chromatography mass spectrometry reveals 14 compounds. The larvicidal effectiveness of CS-fabricated AgNPs was 2.35 μg·mL (I Instar) to 6.24 μg·mL (pupae). The field application in water storage reservoirs of both CS extracts and CS-AgNPs (10 × LC ) led to a 68–69% reduction in larval density after 72 h post-treatment. Ecotoxicological impact of CS-fabricated AgNPs was evaluated on the predatory efficacy of on all the larval instars and pupae of . Finally, CS-AgNPs were tested to elucidate its anti-biofilm attributes. The CS-AgNPs at 125 μg·mL showed a biofilm inhibition of 90% on and 79% on . These results support the use of CS-AgNPs for futuristic green alternative to mosquito vector management.