Effects of miR-144 on Proliferation, Migration, Invasion and PI3K Pathway of Pancreatic Cancer SW1990 Cells

Objective To investigate the effects of microRNA-144 (miR-144) on the proliferation, migration, invasion and phosphoinositide-3 kinase (PI3K) pathway of pancreatic cancer SW1990 cells. Methods The expression of miR-144 in human normal pancreatic epithelial cell line HPDE and pancreatic cancer cells SW1990 were detected by RT-qPCR. SW1990 cells were transfected with miR-144 negative control plasmid (negative transfection group) and miR-144 mimic plasmid (miR-144 overexpression group), and untreated cells were taken as blank control group. The expression level of miR-144 in each group was detected by RTqPCR. CCK-8 assay was used to detect cell proliferation. Plate clone formation assay was used to detect colony formation. Transwell assay was used to detect cell migration and invasion. The expression levels of PI3K and phosphorylated protein (p-PI3K), serine-threonine protein kinase (Akt) and phosphorylated protein (p-Akt) proteins in PI3K pathway were detected by Western blot. Results The expression level of mi