Fast saccharide mapping method for quality consistency evaluation of commercial xylooligosaccharides collected in China

Due to the extensive use of xylooligosaccharides (XOS) as functional food ingredients, many inferior goods and even adulterants are generally found in the market, which may pose a health hazard to certain populations. Chromatography method such as high-performance liquid chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) is traditionally applied for the quality analysis of XOS. However, it is time consuming due to the prolonged separation and pre- or post- derivatization procedure. In this study, a fast saccharide mapping method based on matrix-assisted laser desorption/time-of-flight mass spectrometry (MALDI-TOF-MS) was developed for the quality consistency analysis of 22 batches of XOS collected from different manufacturers in China. The time needed for saccharides analysis using MALDI-MS was less than 30 min for one plate, at least 6 times faster than that by the traditional HPTLC chromatography method. In addition, MALDI-MS possessed higher resolution for XOS with DP4-DP7 based on the difference of m/z, which is hardly separated using HPTLC. The results showed that XOS were present only in samples XY01-XY11, samples XY12-XY14 only consisted of hex oligosaccharides, and samples XY15-XY22 were free of oligosaccharides. These indicate that the quality consistency of XOS products in the China market was poor, which should be carefully investigated. •Fast saccharide mapping method was developed based on MADLI-TOF-MS.•Quality consistency of commercial xylooligosaccharides collected in China was evaluated.•Glycosidic linkage analysis was also used for identification of xylooligosaccharides.•Fifty percent of commercial xylooligosaccharides are mislabeled.