Generation of a homozygous HDAC6 knockout human embryonic stem cell line by CRISPR/Cas9 editing

•HDAC6 plays important roles in several biological processes.•HDAC6 is an attractive therapeutic target for a variety of diseases.•CRISPR/Cas9 is an effective method of gene editing in human embryonic stem cells.•We produced a homozygous HDAC6-knockout human embryonic stem cell line: WAe009-A-21. Histone deacetylase 6 (HDAC6) is a unique cytoplasmic enzyme in the HDAC family. The HDAC6 has been shown to play important roles in several biological processes. Meanwhile, it is also an attractive therapeutic target for a variety of diseases. However, the mechanism of HDAC6 function is not fully understood yet, and it is still lacking highly specific targeted drugs. Here, we generated a homozygous HDAC6 knockout human embryonic stem cell (hESC) line, WAe009-A-21 by the CRISPR/Cas9-based gene editing method. The WAe009-A-21 cell line does not express HDAC6 protein, while maintaining normal 46, XX karyotype, pluripotency, and trilineage differentiation potential.