Thin-layer chromatographic separation of sulfated and nonsulfated lithocholic acids and their glycine and taurine conjugates

A method for superior thin-layer chromatographic separation of lithocholic acid and its N-glycine and N-taurine conjugates, as well as their respective 3alpha-sulfates, is described. A solvent system of chloroform-methanol-acetic acid-water 65:24:15:9 (v/v) is used with air-dried plates of silicic a...

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Veröffentlicht in:	Journal of lipid research 1975-03, Vol.16 (2), p.159-160
Hauptverfasser:	Cass, O W, Cowen, A E, Hofmann, A F, Coffin, S B
Format:	Artikel
Sprache:	eng
Schlagworte:	Cholic Acids - isolation & purification Chromatography, Thin Layer Evaluation Studies as Topic Glycine - isolation & purification Lithocholic Acid - isolation & purification Sulfuric Acids - isolation & purification Taurine - isolation & purification
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Zusammenfassung:	A method for superior thin-layer chromatographic separation of lithocholic acid and its N-glycine and N-taurine conjugates, as well as their respective 3alpha-sulfates, is described. A solvent system of chloroform-methanol-acetic acid-water 65:24:15:9 (v/v) is used with air-dried plates of silicic acid containing calcium sulfate (10% by weight) under conditions of chamber saturation.
ISSN:	0022-2275
DOI:	10.1016/S0022-2275(20)36743-2