(1' S )-1'-Acetoxyeugenol Acetate Enhances Glucose-Stimulated Insulin Secretion

have been widely used as spice or traditional medicine in East Asia, commonly known as Thai ginger. In the present study, seven major phenylpropanoids, (±)-1'-hydoxychavicol acetate ( ; HCA), (1' )-1'-acetoxychavicol acetate ( ; ACA), (1' )-1'-acetoxyeugenol acetate ( ; AEA), eugenyl acetate ( ), - -coumaraldehyde ( ), - -acetoxycinnamyl alcohol ( ), and - -coumaryl diacetate ( ), were isolated from the 95% EtOH and hot water extracts of the rhizomes of . by chromatographic method. Phenylpropanoids - were evaluated for glucose-stimulated insulin secretion (GSIS) effect and α-glucosidase inhibitory activity. Phenylpropanoids - increase GSIS effect without cytotoxicity in rat INS-1 pancreatic β-cells. In addition, INS-1 cells were treated with AEA ( ) to determine a plausible mechanism of β-cell function and insulin secretion through determining the activation of insulin receptor substrate-2 (IRS-2), phosphatidylinositol 3-kinase (PI3K), Akt, and pancreatic and duodenal homeobox-1 (PDX-1). Upon treatment with AEA ( ), INS-1 cells showed an increase in these protein expressions. Meanwhile, AEA ( ) exhibited α-glucosidase inhibitory activity. On the basis of the above findings, we suggest AEA ( ) as a potential antidiabetic agent.