Molecular identification of Haemonchus contortus in sheep from Upper Egypt

spp. are considered the most important strongylid nematodes with a worldwide distribution. The parasite's blood-sucking nature can lead to severe anemia in infected animals. Despite its widespread impact, there is a dearth of comprehensive data on morphological and molecular identification methods for spp. in sheep from Upper Egypt. To address this gap, our current study aimed to assess the prevalence of spp. in 400 sheep fecal samples. We employed microscopic examination and molecular techniques, using polymerase chain reaction (PCR) targeting the 18S gene for precise identification. Additionally, the potential risk factors associated with the infection by the parasite in sheep were explored. The study pointed out that 33.00% (132 of 400) of the examined sheep were infected with spp. Sheep age and seasonal variability were found to be significant factors ( 0.05) was found between the sex of the examined sheep and the occurrence of haemonchosis. Employing molecular methods, we isolated and identified the parasite through PCR analysis of cultured larvae, which were then subsequently confirmed as Haemonchus contortus via phylogenetic analysis. The study concluded that there was a relatively high occurrence of s among sheep from Upper Egypt. We recommend the implementation of stringent and effective control measures to combat the infection and safeguard livestock health.