Association of 25 (OH)-D3 Level With the CYP27B1 rs10877012 Variants and Metabolic Parameters in T2DM Patients From Kurdistan, Iraq

Background:There is an increasing prevalence of type 2 diabetes mellitus (T2DM) worldwide. The deficiency of vitamin D is a worldwide health problem that can increase susceptibility to T2DM. Cytochrome P450 family 27 subfamily B member 1 (CYP27B1) encodes the enzyme 1α-hydroxylase in the kidney, which converts 25 (OH)-D3 to 1,25 (OH)2-D3. The CYP27B1 rs10877012 G/T polymorphism is located in the gene promoter and can influence vitamin D3 level. Objectives: This study aimed to investigate the relationship between CYP27B1 gene polymorphism and the 25 (OH)-D3 serum level and the risk of T2DM. Additionally, the effect of 25 (OH)-D3 level on metabolic parameters was investigated. Methods: We investigated 310 individuals including 205 T2DM patients and 105 healthy subjects from the city of AL-Sulaymaniyah, Kurdistan of Iraq. The CYP27B1 gene variants were identified by the polymerase chain reaction followed by digestion with HinfI restriction enzyme. Results: The mean level of 25 (OH)-D3 was 18.4±8.5 ng/mL in T2DM patients and 24.1±9.3 ng/mL in healthy controls (P