Antibacterial and Cytotoxicity characteristics of experimental epoxy -based endodontic sealer loaded with silver gold nanoparticles: in vitro study

Background Enhancing the antibacterial capabilities of dental materials by adding nanoparticles has been the subject of some research. However, the potential toxic effect of this material on the vital tissues should be investigated to avoid additional damage to the tissue. Objective This study aimed to validate the long-term cytotoxic and antibacterial properties of an epoxy resin-based endodontic sealer (AH Plus) with and without loading with silver gold nanoparticles (Nano Care Plus Silver Gold®). Material and methods The tested groups were Nano Care Gold (group I), modified resin sealer (m AH Plus; group II) and AH Plus served as a control group (group III). Agar diffusion was used to measure the antibacterial activity against Enterococcus faecalis . Using the MTT test, cytotoxicity assessment was carried out in accordance with ISO-10993-5 guidelines to assess the cells’ viability as soon as possible and after two and four weeks. The t-test was used to statistically examine the data. The chosen significance threshold was P