Helium/Argon-Generated Cold Atmospheric Plasma Facilitates Cutaneous Wound Healing

Cold atmospheric plasma jet (CAPJ) or non-thermal plasma jet has been employed in various biomedical applications based on their functions in bactericidal activity and wound healing. However, the effect of CAPJ generated by a particular composition of gases on wound closure and the underlying mechan...

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Veröffentlicht in:Frontiers in bioengineering and biotechnology 2020-06, Vol.8, p.683-683
Hauptverfasser: Lou, Bih-Show, Hsieh, Jang-Hsing, Chen, Chun-Ming, Hou, Chun-Wei, Wu, Hui-Yu, Chou, Pang-Yun, Lai, Chih-Ho, Lee, Jyh-Wei
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Sprache:eng
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Zusammenfassung:Cold atmospheric plasma jet (CAPJ) or non-thermal plasma jet has been employed in various biomedical applications based on their functions in bactericidal activity and wound healing. However, the effect of CAPJ generated by a particular composition of gases on wound closure and the underlying mechanisms that regulate wound healing signals remain elusive. In the present study, we investigated the impact of helium (He)- or a gas mixture of He and argon (He/Ar)-generated CAPJ on cell proliferation, which is a pivotal step during the wound healing process. With careful treatment duration control, He/Ar-CAPJ effectively induced keratinocyte proliferation and migration mediated through the activation of epithelial-to-mesenchymal transition (EMT) and cell cycle progression, which was evidenced by a decrease in E-cadherin levels and increases in N-cadherin, cyclin D1, Ki-67, Cdk2, and p-ERK levels. Rat wound healing studies showed that He/Ar-CAPJ treatment facilitated granulation tissue formation and mitigated inflammation in cutaneous tissue, resulting in accelerated wound closure. These findings highlight the possibility that He/Ar-CAPJ can be developed as a therapeutic agent for enhancing wound healing.
ISSN:2296-4185
2296-4185
DOI:10.3389/fbioe.2020.00683