Effect of Tween 80 and Acetone on the Secretion, 
Structure and Antioxidant Activities of Exopolysaccharides from Lentinus tigrinus

Effect of Tween 80 and Acetone on the Secretion, 
Structure and Antioxidant Activities of Exopolysaccharides from Lentinus tigrinus

In this study, the effects of the addition of Tween 80 and acetone on secretion, structure and antioxidant activities of exopolysaccharides (EPS) were investigated. It was found that Tween 80 and acetone displayed a stimulatory effect on EPS secretion. The EPS obtained by the addition of Tween 80 (E...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Food technology and biotechnology 2016, Vol.54 (3), p.290-295
Hauptverfasser: He, Peixin, Wu, Shuangshuang, Pan, Lige, Sun, Siwen, Mao, Duobin, Xu, Chunping
Format: Artikel
Sprache:eng
Schlagworte:
Acetone
Antioxidants
antiproliferative activity
Aqueous solutions
Basidiomycota
Biological activity
Biosynthesis
Chemical properties
Chromatography
Ethanol
exopolysaccharides
Fourier transforms
Glucose
Lentinus tigrinus
molecular conformation
molecular mass
Observations
Original Scientific Papers
Permeability
Polysaccharides
Properties
Solvents
Surface active agents
Surfactants
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:In this study, the effects of the addition of Tween 80 and acetone on secretion, structure and antioxidant activities of exopolysaccharides (EPS) were investigated. It was found that Tween 80 and acetone displayed a stimulatory effect on EPS secretion. The EPS obtained by the addition of Tween 80 (EPS-T), acetone (EPS-A) and control (EPS--C) were purified by Sepharose CL-6B gel filtration chromatography and molecular mass of purified fractions was estimated to be 22.1, 137 and 12 kDa, respectively. Monosaccharide composition analysis indicated that EPS-T, EPS-A and EPS-C were mainly composed of glucose and mannose. Congo Red test indicated that EPS-T and EPS-A had a highly ordered conformation of triple helix, while EPS-C had a random coil conformation. Furthermore, EPS-A exhibited higher DPPH scavenging and antiproliferative activities than EPS--C and EPS-T, which might be attributed to the molecular mass.
ISSN:1330-9862
1334-2606
DOI:10.17113/ftb.54.03.16.4211