Enhancing Algal Yield and Nutrient Removal from Anaerobic Digestion Piggery Effluent by an Integrated Process-Optimization Strategy of Fungal Decolorization and Microalgae Cultivation

The dark brown anaerobic digestion piggery effluent (ADPE) with a large amount of ammonium generally needs high dilution before microalgae cultivation due to its inhibiting effects on algal growth. Due to the strong decolorization of fungi by degrading organic compounds in wastewater, the process-optimization integrated strategy of fungal decolorization of ADPE and subsequent microalgae cultivation with ammonium-tolerant strain may be a more reliable procedure to reduce the dilution ratio and enhance algal biomass production, and nutrient removal from ADPE. This study determined a suitable fungal strain for ADPE decolorization, which was isolated and screened from a local biogas plant, and identified using 26s rRNA gene sequence analysis. Subsequently, ADPE was pretreated by fungal decolorization to make low-diluted ADPE suitable for the algal growth, and conditions of microalgae cultivation were optimized to achieve maximum algal yield and nutrient removal from the pretreated ADPE. The results showed one promising locally isolated fungal strain, Nanchang University-27, which was selected out of three candidates and identified as Lichtheimia ornata, presenting a high decolorization to ADPE through fungal pretreatment. Five-fold low-diluted ADPE pretreated by L. ornata was the most suitable medium for the algal growth at an initial concentration of ammonium nitrogen of 380 mg L−1 in all dilution treatments. Initial optical density of 0.3 and pH of 9.0 were optimal culture conditions for the algal strain to provide the maximum algal yield (optical density = 2.1) and nutrient removal (88%, 58%, 65%, and 77% for the removal rates of ammonium nitrogen, total nitrogen, total phosphorus, and chemical oxygen demand, respectively) from the pretreated ADPE. This study demonstrated that fungal decolorization and subsequent microalgae cultivation could be a promising approach to algal biomass production and nutrient removal from ADPE.