Properties of Camphorated Paramonochlorophenol and Camphorated Paramonochlorophenol Associated to Calcium Hydroxide

Objective: To evaluate the biocompatibility of camphoratedparamonochlorophenol (CMCP) at a proportion of 3.5:6.5 andcamphorate paramonochlorophenol associated to calcium hydroxide(CMCP+Ca(OH)2) in rat subcutaneous tissue, and the antimicrobialpotential of these medications against some microorganisms.Method: Ten Wistar rats were anesthetized and received anintravenous injection of Evan’s blue dye followed by inoculation of0.1 mL of the tested drugs in the dorsal region. The amount ofreleased dye bound to plasma proteins was evaluated underspectrophotometry. The antimicrobial activity of the substancesagainst S. aureus, S. mutans, B. subtilis and C. albicans wasverified by measuring the zones of bacterial growth inhibition usingthe agar diffusion test.Results: The mean optical density values for CMCP andCMCP+Ca(OH)2 were 0.325±0.025 (moderate reaction) and0.211±0.040 (mild reaction), respectively. The measurements ofthe zones of bacterial growth inhibition were as follows: CMCP =7.29 mm and CMCP+Ca(OH)2 = 7.29 mm for S. aureus; CMCP = 8.57mm and CMCP+Ca(OH)2 = 7.0 mm for S. mutans; CMCP = 8.57 mmand CMCP+Ca(OH)2 = 7.43 mm for B. subtilis; and CMCP - 8.71 mmand CMCP+Ca(OH)2 - 8.29 mm para C. albicans.Conclusion: The obtained data indicated that CMCP was moreirritating than CMCP+Ca(OH)2, while no statistically significantdifference was observed between the tested substances regardingtheir antimicrobial activity (ANOVA).