Haemopedia: An Expression Atlas of Murine Hematopoietic Cells

Hematopoiesis is a multistage process involving the differentiation of stem and progenitor cells into distinct mature cell lineages. Here we present Haemopedia, an atlas of murine gene-expression data containing 54 hematopoietic cell types, covering all the mature lineages in hematopoiesis. We include rare cell populations such as eosinophils, mast cells, basophils, and megakaryocytes, and a broad collection of progenitor and stem cells. We show that lineage branching and maturation during hematopoiesis can be reconstructed using the expression patterns of small sets of genes. We also have identified genes with enriched expression in each of the mature blood cell lineages, many of which show conserved lineage-enriched expression in human hematopoiesis. We have created an online web portal called Haemosphere to make analyses of Haemopedia and other blood cell transcriptional datasets easier. This resource provides simple tools to interrogate gene-expression-based relationships between hematopoietic cell types and genes of interest. [Display omitted] •Haemopedia is an expression dataset covering 54 murine hematopoietic cell types•Relationship of cells recreated with the expression pattern of small sets of genes•Many murine lineage-specific genes still lineage specific in humans•Data can be accessed via online portal for ease of analysis De Graaf and colleagues transcriptionally profile 54 murine blood cell types from stem to mature cells. Lineage relationship of cells can be reconstructed from the expression patterns of small sets of genes. Lineage-specific genes are identified, many of which show conserved expression patterns in humans. An online web portal to this dataset is provided for ease of analysis.