A dual Ucp1 reporter mouse model for imaging and quantitation of brown and brite fat recruitment
Brown adipose tissue (BAT) dissipates nutritional energy as heat through uncoupling protein 1 (UCP1). The discovery of functional BAT in healthy adult humans has promoted the search for pharmacological interventions to recruit and activate brown fat as a treatment of obesity and diabetes type II. Th...
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Veröffentlicht in: | Molecular metabolism (Germany) 2019-02, Vol.20, p.14-27 |
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Zusammenfassung: | Brown adipose tissue (BAT) dissipates nutritional energy as heat through uncoupling protein 1 (UCP1). The discovery of functional BAT in healthy adult humans has promoted the search for pharmacological interventions to recruit and activate brown fat as a treatment of obesity and diabetes type II. These efforts require in vivo models to compare the efficacy of novel compounds in a relevant physiological context.
We generated a knock-in mouse line expressing firefly luciferase and near-infrared red florescent protein (iRFP713) driven by the regulatory elements of the endogenous Ucp1 gene.
Our detailed characterization revealed that firefly luciferase activity faithfully reports endogenous Ucp1 gene expression in response to physiological and pharmacological stimuli. The iRFP713 fluorescence signal was detected in the interscapular BAT region of cold-exposed reporter mice in an allele-dosage dependent manner. Using this reporter mouse model, we detected a higher browning capacity in female peri-ovarian white adipose tissue compared to male epididymal WAT, which we further corroborated by molecular and morphological features. In situ imaging detected a strong luciferase activity signal in a previously unappreciated adipose tissue depot adjunct to the femoral muscle, now adopted as femoral brown adipose tissue. In addition, screening cultured adipocytes by bioluminescence imaging identified the selective Salt-Inducible Kinase inhibitor, HG-9-91-01, to increase Ucp1 gene expression and mitochondrial respiration in brown and brite adipocytes.
In our mouse model, firefly luciferase activity serves as a bona fide reporter for dynamic regulation of Ucp1. In addition, by means of iRFP713 we are able to monitor Ucp1 expression in a non-invasive fashion.
•A new reporter mouse model enables bona fide monitoring of endogenous Ucp1 gene expression.•The new generation near-infrared fluorescent protein iRFP713 enables noninvasive imaging of brown fat.•Reporter mice show sex-specific differences in browning propensity of gonadal WAT.•We discovered a novel brown adipose tissue depot, now adopted as femoral brown fat.•Our reporter cell assays revealed the pan-Salt-Inducible Kinase as an inhibitor of Ucp1 gene expression. |
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ISSN: | 2212-8778 2212-8778 |
DOI: | 10.1016/j.molmet.2018.11.009 |