Detection of telomerase activity in Plasmodium falciparum using a nonradioactive method

A simple, quick and sensitive method was used to detect telomerase activity in Plasmodium falciparum. The telomeric repeat amplification protocol (TRAP assay) was modified using electrophoresis and staining with SYBR-green I to detect telomerase activity in a range of 10 to 10(7) parasites. This mig...

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Veröffentlicht in:	Memórias do Instituto Oswaldo Cruz 2003-07, Vol.98 (5), p.693-695
Hauptverfasser:	Rubiano, Claudia C, Wasserman, Moises
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals assay Electrophoresis, Polyacrylamide Gel malaria nonradioactive PARASITOLOGY Plasmodium Plasmodium falciparum - enzymology Polymerase Chain Reaction - methods Repetitive Sequences, Nucleic Acid telomerase Telomerase - metabolism Telomere - genetics TROPICAL MEDICINE
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Zusammenfassung:	A simple, quick and sensitive method was used to detect telomerase activity in Plasmodium falciparum. The telomeric repeat amplification protocol (TRAP assay) was modified using electrophoresis and staining with SYBR-green I to detect telomerase activity in a range of 10 to 10(7) parasites. This might be a useful way to ascertain telomerase activity in different types of nontumor cells.
ISSN:	0074-0276 1678-8060 0074-0276 1678-8060
DOI:	10.1590/S0074-02762003000500018