AKTIVITAS HIDROLISIS ENZIM LIPASE DARI KENTOS KELAPA TERHADAP MINYAK KELAPA Hidrolysis Activity of Lipase Enzyme from Coconut Houstorium for Coconut Oil
This research was aimed to study hydrolysis conditions of houstorium lipases enzyme using coconut oil as substrate. Hydrolysis conditions studied were substrate (coconut oil) concentration, enzyme substrate ratio, duration of hydro- lysis and effect of stirring to hydrolysis. The results show that...
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Veröffentlicht in: | Agritech 2012-05, Vol.30 (3) |
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Hauptverfasser: | , , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | This research was aimed to study hydrolysis conditions of houstorium lipases enzyme using coconut oil as substrate. Hydrolysis conditions studied were substrate (coconut oil) concentration, enzyme substrate ratio, duration of hydro- lysis and effect of stirring to hydrolysis. The results show that lipase of coconut houstorium may be optimally used at a coconut oil concentration of 10 %, enzyme to substrate ratio of 3 : 10 (v/v) and hydrolysis for 60 minutes with stirring. ABSTRAK Penelitian ini mempelajari kondisi hidrolisis minyak kelapa yang optimum menggunakan enzim lipase dari kentos kelapa. Kondisi hidrolisis yang dipelajari meliputi konsentrasi substrat optium, perbandingan enzim : substrat dan lama hidrolisis yang optimum serta pengaruh pengadukan selama hidrolisis. Hasil penelitian menunjukkan bahwa, hidrolisis minyak kelapa menggunakan enzim lipase kentos kelapa menghasilkan asam lemak bebas paling banyak pada kon- sentrasi substrat (minyak kelapa) 10 %, perbandingan enzim : substrat yaitu 3 : 10 (v/v), lama hidroloisa 60 menit dan dilakukan pengadukan selama hidrolisis. |
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ISSN: | 0216-0455 2527-3825 |
DOI: | 10.22146/agritech.9669 |