Enzymatic aqueous technology for simultaneous coconut protein and oil extraction
In this study, the following commercial enzymes were evaluated in the enzymatic extraction of oil and protein from coconut: Celluclast, Termamyl, Viscozyme, Neutrase y Protease. Preliminary experiments were carried out for selection of enzymes, enzyme concentration and incubation time. In order to v...
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Veröffentlicht in: | Grasas y aceites (Sevilla) 2003-01, Vol.54 (1), p.77-80 |
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Sprache: | eng |
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Zusammenfassung: | In this study, the following commercial enzymes were evaluated in the enzymatic extraction of oil and protein from coconut: Celluclast, Termamyl, Viscozyme, Neutrase y Protease. Preliminary experiments were carried out for selection of enzymes, enzyme concentration and incubation time. In order to verify the effects of pH and water/substrate ratio, a response surface methodology was applied based in a 32 full factorial experimental design. The pH is the most meaningful parameter on oil and protein extraction yields, with a significance level higher than 90%. The maximal extraction yields of oil and protein emulsion (83%) was reached using Viscozyme L and subsequently Neutrase 1.5 MG at concentrations of 0.6% (w/w) and 0.3% (w/w), respectively, total incubation time of 60 minutes, substrate/water ratio 1:6 and pH around 7 (no adjustment). |
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ISSN: | 0017-3495 1988-4214 |
DOI: | 10.3989/gya.2003.v54.i1.281 |