A review on label free biosensors

Label-free biosensing has advanced significantly in recent years due to its capacity for quick and inexpensive bio-detection in small volumes. Additionally, they have developed into lab-on-a-chip technology and may be able to perform real-time analysis. In this regard, we have talked about label-free bio sensing based on a range of transducers, operational concepts, novel nanomaterial, device fabrication, and application. Recent developments in label-free biosensor technology, including aptamer-based sensors, field-effect transistor-based biosensors, optical-based biosensors, SPR, LSPR-based biosensors, and paper biosensors based on nanobiotechnology, are highlighted. It is intended to give the reader a reason to peep into the planet of label-free biosensors. •We discussed point of care sensing device developed using different Nanoparticles in label free way.•This review give clear idea of biosensor and its components, Working principle and its efficiency to sense target analytes.•The recent successfully established label-free biosensors based on operation, Probe and substrate are discussed.•The article highlights innovations in microfluidics and optical sensors that address the growing demand for food safety.