Loss of functional cryptochrome 1 reduces robustness of 24-hour behavioral rhythms in monarch butterflies

Light is one of the strongest cues for entrainment of circadian clocks. While some insect species rely only on visual input, others like Drosophila melanogaster use both the visual system and the deep-brain blue-light photoreceptor cryptochrome for entraining circadian rhythms. Here, we used the monarch butterfly Danaus plexippus (dp), which possesses a light-sensitive cryptochrome 1 (dpCry1), to test the conservation of mechanisms of clock entrainment. We showed that loss of functional dpCry1 reduced the amplitude and altered the phase of adult eclosion rhythms, and disrupted brain molecular circadian rhythms. Robust rhythms could be restored by entrainment to temperature cycles, indicating a likely functional core circadian clock in dpCry1 mutants. We also showed that rhythmic flight activity was less robust in dpCry1 mutants, and that visual impairment in dpNinaB1 mutants impacted flight suppression at night. Our data suggest that dpCRY1 is a major photoreceptor for light-entrainment of the monarch circadian clock. [Display omitted] •Monarch dpCry1 mutants display damped eclosion and brain molecular circadian rhythms•Temperature cycles re-entrain eclosion and molecular rhythms in dpCry1 mutants•Robustness of rhythmic adult flight activity is decreased in dpCry1 mutants•Genetic impairment of opsin-based photoreception impacts flight suppression at night Entomology; Natural sciences; Neuroscience; Zoology