Effects of Desflurane exposure and Laparotomy on genomic biomarkers and hepatic histopathology in an experimentally induced liver injury model: A pilot study

Halogenated anesthetics are known to cause hepatotoxicity that can be evaluated by the emerging genomic biomarkers, that is, miRNA-122 and miRNA-192. Thirty Wister rats were distributed randomly in five equal groups; Control Group (group C); Desflurane Group (group D); Hypoxia Group (group H); Hypoxia + Desflurane Group (group HD); and Hypoxia + Desflurane + Laparotomy Group (group HDL). Animals in the Groups D, HD, HDL were exposed to 6% desflurane. Rats were exposed to hypoxic mixture in Groups H, HD, and HDL. Animals in Group HDL were additionally subjected to laparotomy with liver palpation. After 24 hours, plasma ALT, AST, GGT, blood and tissue miR-122, and miR-192 gene expression and histopathological examination of liver biopsies were performed. Plasma and tissue miR122 and miR192 showed significant increase ascendingly in H, D, HD, HDL groups (p value