cDNA-AFLP display for the isolation of Peronospora parasitica genes expressed during infection in Arabidopsis thaliana

To identify genes from the obligatory biotrophic oomycete Peronospora parasitica that are expressed during infection in Arabidopsis thaliana we employed cDNA-amplified fragment length polymorphism (AFLP) display. cDNA-AFLP fragments from infected and non-infected leaves were separated in parallel by...

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Veröffentlicht in:	Molecular plant-microbe interactions 2000-08, Vol.13 (8), p.895-898
Hauptverfasser:	VAN DER BIEZEN, E. A, JUWANA, H, PARKER, J. E, JONES, J. D. G
Format:	Artikel
Sprache:	eng
Schlagworte:	Arabidopsis - microbiology Arabidopsis thaliana Base Sequence Biological and medical sciences differential display DNA Primers DNA, Complementary Fundamental and applied biological sciences. Psychology Fungal plant pathogens Gene Expression Regulation, Fungal Gene Expression Regulation, Plant Genes, Fungal Molecular Sequence Data Nucleic Acid Amplification Techniques Oomycetes - genetics Peronospora parasitica Phytopathology. Animal pests. Plant and forest protection Variation, races, biotypes, parasitic specialization, genetics
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Zusammenfassung:	To identify genes from the obligatory biotrophic oomycete Peronospora parasitica that are expressed during infection in Arabidopsis thaliana we employed cDNA-amplified fragment length polymorphism (AFLP) display. cDNA-AFLP fragments from infected and non-infected leaves were separated in parallel by gel electrophoresis and displayed by autoradiography. Most differential gene fragments were derived from P. parasitica.
ISSN:	0894-0282 1943-7706
DOI:	10.1094/MPMI.2000.13.8.895