p53 activation: a checkpoint for precision genome editing?

Recent work has reported that the activation of the p53 pathway in primary cells hampers the efficiency of genome editing by CRISPR/Cas9 via induction of apoptosis or cell cycle arrest. These findings highlight the importance of wholly comprehending cellular responses to programmable nucleases in th...

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Veröffentlicht in:	Genome medicine 2018-08, Vol.10 (1), p.66-66, Article 66
Hauptverfasser:	Conti, Anastasia, Di Micco, Raffaella
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Apoptosis Cancer Cell cycle Comment CRISPR CRISPR-Cas Systems Deoxyribonucleic acid DNA DNA damage Efficiency Epithelial cells Gene Editing - methods Gene mutation Genes Genetic engineering Genome editing Genomes Humans Nuclease p53 Protein Precision Medicine - methods Stem cells Therapeutic applications Tumor Suppressor Protein p53 - genetics Tumor Suppressor Protein p53 - metabolism
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Beschreibung
Zusammenfassung:	Recent work has reported that the activation of the p53 pathway in primary cells hampers the efficiency of genome editing by CRISPR/Cas9 via induction of apoptosis or cell cycle arrest. These findings highlight the importance of wholly comprehending cellular responses to programmable nucleases in therapeutically relevant cell types to fulfill the promise of genome editing for therapeutic applications for human diseases.
ISSN:	1756-994X 1756-994X
DOI:	10.1186/s13073-018-0578-6