Physiology and transcriptomics highlight the underlying mechanism of sunflower responses to drought stress and rehydration

Drought can adversely influence the crop growth and production. Accordingly, sunflowers have strong adaptability to drought; hence, we conducted analyses for sunflower seedlings with drought stress and rehydration drought acclimation through physiological measurements and transcriptomics. It showed that drought can cause the accumulation of ROS and enhance the activity of antioxidant enzymes and the content of osmolytes. After rehydration, the contents of ROS and MDA were significantly reduced concomitant with increased antioxidant activity and osmotic adjustment. Totally, 2,589 DEGs were identified among treatments. Functional enrichment analysis showed that DEGs were mainly involved in plant hormone signal transduction, MAPK signaling, and biosynthesis of secondary metabolites. Comparison between differentially spliced genes and DEGs indicated that bHLH025, NAC53, and SINAT3 may be pivotal genes involved in sunflower drought resistance. Our results not only highlight the underlying mechanism of drought stress and rehydration in sunflower but also provide a theoretical basis for crop genetic breeding. [Display omitted] •Rehydration can alleviate ROS-damaged sunflowers accumulated by drought stress•DEGs in hormone, MAPK, and secondary metabolite pathways affect drought resistance•bHLH025, NAC53, and SINAT3 may play key roles in sunflower drought resistance•ROS scavenging, MAPK signaling, SMs, and AS aid in sunflower drought resistance Plant biology; Plant physiology; Transcriptomics