The pathogenicity of vancomycin-resistant Enterococcus faecalis to colon cancer cells

The aim of this study was to investigate the pathogenicity of vancomycin-resistant Enterococcus faecalis (VREs) to human colon cells in vitro. Three E. faecalis isolates (2 VREs and E. faecalis ATCC 29212) were cocultured with NCM460, HT-29 and HCT116 cells. Changes in cell morphology and bacterial...

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Veröffentlicht in:	BMC infectious diseases 2024-02, Vol.24 (1), p.230-230, Article 230
Hauptverfasser:	Zhang, Li, Deng, Mingxia, Liu, Jing, Zhang, Jiajie, Wang, Fangyu, Yu, Wei
Format:	Artikel
Sprache:	eng
Schlagworte:	1-Phosphatidylinositol 3-kinase Adhesion AKT protein Angiogenesis Antibiotic resistance Antibodies Bacteria Cancer Care and treatment Cell adhesion & migration Cell culture Cell migration Cell morphology Colon cancer Colonic Neoplasms Colorectal cancer Complications and side effects Cytology Development and progression Drug resistance in microorganisms Endothelial cells Endothelium Enterococcal infections Enterococcus faecalis Enzyme-linked immunosorbent assay Enzymes Ethylenediaminetetraacetic acid Growth factors Health aspects Host-bacteria relationships Human Umbilical Vein Endothelial Cells - metabolism Humans IL-8 In vitro methods and tests Inflammation Interleukin 8 Interleukins Microbiota Morphology Multiplicity of infection Neovascularization Nosocomial infections Pathogenicity Pathogens Phosphatidylinositol 3-Kinases - metabolism PI3K/AKT/mTOR signaling pathway Proteins Proto-Oncogene Proteins c-akt - metabolism Signal transduction Software TOR protein TOR Serine-Threonine Kinases - metabolism Umbilical vein Vancomycin Vancomycin - pharmacology Vancomycin-Resistant Enterococci Variance analysis Vascular endothelial growth factor Vascular Endothelial Growth Factor A - genetics VEGFA Virulence Western blotting
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Beschreibung
Zusammenfassung:	The aim of this study was to investigate the pathogenicity of vancomycin-resistant Enterococcus faecalis (VREs) to human colon cells in vitro. Three E. faecalis isolates (2 VREs and E. faecalis ATCC 29212) were cocultured with NCM460, HT-29 and HCT116 cells. Changes in cell morphology and bacterial adhesion were assessed at different time points. Interleukin-8 (IL-8) and vascular endothelial growth factor A (VEGFA) expression were measured via RT-qPCR and enzyme-linked immunosorbent assay (ELISA), respectively. Cell migration and human umbilical vein endothelial cells (HUVECs) tube formation assays were used for angiogenesis studies. The activity of PI3K/AKT/mTOR signaling pathway was measured by Western blotting. The growth and adhesion of E. faecalis at a multiplicity of infection (MOI) of 1:1 were greater than those at a MOI of 100:1(p
ISSN:	1471-2334 1471-2334
DOI:	10.1186/s12879-024-09133-2