Platycodon D attenuates renal ischemia-reperfusion injury in rats

Objective To investigate the protective effect of Platycodin D (PD) on renal ischemia-reperfusion injury in rats. Methods The rats were divided into sham group, model group, low, middle and high-dose groups with 10 animals in each. The rat model of ischemia reperfusion injury was established by clamping the bilateral renal pedicles in model group, low, middle and high-dose group. The rats in sham group underwent the same modeling operation but did not clamp the renal pedicles. Before 5 minutes of modeling, the rats of low, middle and high-dose group were intra-peritoneally injected with 12.5, 25 and 50 mg/kg PD, respectively. After 24 h of modeling, the serum creatinine (Cr) and blood urea nitrogen (BUN) levels, as well as antioxidant markers[superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA)] levels in kidney tissues were measured. HE staining was used to evaluate renal tissue lesions. Immuno-histochemical staining was used to detect the expression of caspase-3 in renal tis