Vehicle transmission of antibiotic-resistant pathogens mediated by plastic debris in aquatic ecosystems

Plastic materials are emerging environmental pollutants acting as potential vehicles for accumulation and spread of multidrug-resistant bacteria. The current study investigates the role of plastics in favoring the dispersal of specific pathogens and their associated antibiotic resistant genes (ARGs). Artificial plastic substrates (APSs) were submerged in seven sampling points of Lake Bracciano (Italy), and after one-month both APSs and raw water (RW) samples were collected. Through the combination of standard microbiological and biochemical techniques, 272 bacterial strains were identified and characterized for antibiotic resistant profiling. Our results revealed a notable difference in terms of diversity and abundance of pathogenic bacteria recovered from APSs, compared to RW. In addition, higher resistance patterns were detected in APSs isolates, with frequent appearance of relevant ARGs and class 1 integrons. These findings reinforce the idea that plastic materials in aquatic ecosystems serve as a reservoir for superbugs, significantly contributing to the dissemination of ARGs. [Display omitted] •Plastic plays a vital role in disseminating superbugs in aquatic ecosystem•Plastic-associated antibiotic resistant bacteria pose hidden risks for humans•Detection of emerging and re-emerging pathogens in environmental sector is threatening•Improving One-Health prevention by enhancing the detection of AMR in aquatic ecosystem Environment; Microbiology