Analysis of the impact of cryopreservation and theophylline on motility of sperm

Sperm parameters, particularly motility, decrease during cryopreservation. Theophylline generally enhances sperm motility. We analyzed effects of theophylline and freezing on sperm motility. Design: Experimental study. Setting: Private IVF lab. Setting: IVF lab of Mehrgan Hospital. 22–55year-old men participated in this study (30 fresh ejaculation and 8 TESE samples). After sperm analysis, we added theophylline (40mM) to half of our samples as case group to compare motility with the remaining samples as control group. Cryopreservation was performed in two groups. After thawing, motility of both groups was recorded. Furthermore, theophylline (40mM) was applied to both groups after thawing again. After adding theophylline, sperm motility improved significantly in all samples. Sperm motility reduced in control group more than the study group after freeze-thaw procedure (P