An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels

A simple protocol is described for the silver staining of polyacrylamide gradient gels used for the separation of restriction fragments of kinetoplast DNA [schizodeme analysis of trypanosomatids (Morel et al., 1980)]. The method overcomes the problems of non-uniform staining and strong background co...

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Veröffentlicht in:	Memórias do Instituto Oswaldo Cruz 1990-01, Vol.85 (1), p.101-106
Hauptverfasser:	Gonçalves, A M, Nehme, N S, Morel, C M
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals DNA DNA, Circular - analysis DNA, Kinetoplast Electrophoresis, Polyacrylamide Gel - methods PARASITOLOGY protein RNA schizodeme analysis silver staining Staining and Labeling TROPICAL MEDICINE trypanosomatids Trypanosomatina - classification
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Beschreibung
Zusammenfassung:	A simple protocol is described for the silver staining of polyacrylamide gradient gels used for the separation of restriction fragments of kinetoplast DNA [schizodeme analysis of trypanosomatids (Morel et al., 1980)]. The method overcomes the problems of non-uniform staining and strong background color which are frequently encountered when conventional protocols for silver staining of linear gels are applied to gradient gels. The method described has proven to be of general applicability for DNA, RNA and protein separations in gradient gels.
ISSN:	0074-0276 1678-8060 0074-0276 1678-8060
DOI:	10.1590/S0074-02761990000100017