Prospecção de extratos vegetais como coadjuvantes de higiene bucal em cães raça Labrador Retriever

The present study aimed to prospect plant extracts as a therapeutic resource, compared to the oral microbiota of dogs. For this purpose, ethanol extracts from different parts of Anacardium Ocidentalis (cashew tree), Stryphnodendron adstringens (barbatimão), Punica granatum (pomegranate), Uncaria tomentosa (cat's claw), Psidium guajava L. (guava), Momordica charantia L. (melon from São Caetano) and Harpagophytum procumbens (devil's claw), prepared in different concentrations. Microorganisms collected from the oral mucosa of 12 Labrador dogs, maintained under the same management and feeding, were seeded in BHI Agar in a petri dish in which paper disks impregnated with 20µL of each extract were circulated. 0.12% chlorhexidine digluconate solution was used as a positive control. The plates were incubated for 12 hours at 36 ºC. Three swabs were collected for each animal and each experiment was carried out in triplicate. The results of the inhibition halo measurements were subjected to the Kruskall-Wallis analysis of variance, followed by the Dunn test, with a significance level of 5%. Based on the results, pomegranate, guava, and barbatimão extracts were selected, as they were statistically significant at concentrations of 250; 125; 62,6mg/mL which suggests that the three are potential candidates for use in the development of pharmaceutical products for use in oral hygiene of dogs. El objetivo del presente estudio fue buscar extractos de plantas como recurso terapéutico, contra la microbiota oral de los perros. Para ello, se obtuvieron extractos etanolicos de distintas partes de Anacardium Ocidentalis (anacardo), Stryphnodendron adstringens, Punica granatum (granada), Uncaria tomentosa (uña de gato), Psidium guajava L. (guayaba), Momordica charantia L. (melón de San Caetano) y Harpagophytum procumbens (garra del diablo), preparados en diferentes concentraciones. Los microorganismos recolectados de la mucosa oral de 12 perros labrador, mantenidos bajo el mismo manejo y alimentación, se sembraron en Agar BHI en una placa petri en la que se distribuyeron discos de papel impregnados con 20µL de cada extracto en diferentes concentraciones. Se utilizó una solución de digluconato de clorhexidina al 0,12% como control positivo. Se muestrearon tres hisopos de cada animal. Las placas se incubaron durante 12 horas a 36 ºC. Los resultados de las medidas de los halos de inhibición se sometieron al análisis de varianza de Kruskall-Wallis, seguido de la prueba de Dunn, con un