Identification of virulence and antibiotic resistance factors in Arcobacter butzleri isolated from bovine milk by Whole Genome Sequencing

is a pathogenic aerobic bacterium responsible for diarrhea and septicemia in humans. It is frequently isolated from food products of animal origin, including milk and dairy products. To data, few reports are currently available on the genetic characteristics and virulence profiles of . The aim of th...

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Veröffentlicht in:Italian journal of food safety 2019-06, Vol.8 (2), p.7840-7840
Hauptverfasser: Parisi, Antonio, Capozzi, Loredana, Bianco, Angelica, Caruso, Marta, Latorre, Laura, Costa, Antonella, Giannico, Anna, Ridolfi, Donato, Bulzacchelli, Carmela, Santagada, Gianfranco
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Sprache:eng
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Zusammenfassung:is a pathogenic aerobic bacterium responsible for diarrhea and septicemia in humans. It is frequently isolated from food products of animal origin, including milk and dairy products. To data, few reports are currently available on the genetic characteristics and virulence profiles of . The aim of this study was to investigate the genetic diversity and to characterize the virulence and antibiotic resistance profiles of 10 strains isolated from bovine milk samples by Whole Genome Sequence (WGS). Multi-Locus Sequence Typing (MLST) revealed that three isolates belonged to the ST66, two to the ST420 and the remaining five strains to the ST627, ST629, ST630, ST633 and ST637, respectively. The 100% of the strains carried , , , , and virulence factors genes; 60% iroE; 50% ; 10% . Resistome prediction showed a multidrug resistance: 100% of isolates resulted resistant to fluoroquinolones and tetracycline; 90% of strains to rifampicin and cephalosporins and a minor percentage to other antibiotics. Furthermore, the 50% of strains harbored four mutations in gene conferring resistance to isoniazid. The study provided interesting data on the virulence characteristics and on the genetic endowment related to the antimicrobial resistance of isolates from milk. The determination of the STs also added information concerning the genetic variability of this microorganism. To date, a very limited number of studies have been published on the typing of using WGS, so this paper proposes an innovative methodological approach that allows a rapid and complete characterization of pathogenic microorganisms.
ISSN:2239-7132
2239-7132
DOI:10.4081/ijfs.2019.7840