Determination of specific antibodies titre to salmonella enteritidis by elisa technique in several selected flocks of laying hens

In this paper, the antibody titre to Salmonella enteritidis (SE) was examined by the ELISA method in two flocks of laying hens, where during routine bacteriological investigations Salmonellae was never isolated, and in one flock where Colysepticemia was diagnosed and Salmonella isolated accidentally. In the flocks were Salmonellae were not isolated, a titre with a high level of specific antibodies to SE was discovered (15 and 45%), while the flock with accidental findings of SE was poorly positive (5%). These results point to the necessity of introducing serological monitoring to SE so that the infection of salmonella may be discovered early and the prevalence in the flock determined, and also for the purpose of applying adequate measures that could reduce the possibility of secretion of SE through eggs. U ovom radu je dat prikaz utvrdjenih vrednosti titara antitela na Salmonella enteritidis (SE) ELISA tehnikom kod dva jata konzumnih kokosi nosilja kod kojih tokom rutinskih bakterioloskih analiza nisu izolovane salmonele i u jednom jatu u kome su salmonele izolovane u okolnostima poremecaja opsteg zdravstvenog stanja. U jatima, u kojima salmonele nisu izolovane ustanovljeni su visoki titri specificnih antitela na SE (45% i 15%), dok je jato sa slucajnim nalazom SE bilo slabo pozitivno (5%). Ovi rezultati ukazuju na neophodnost uvodjenja seroloskog monitoringa na SE kako bi na vreme mogla da se otkrije infekcija izazvana salmonelama i proceni njena rasprostranjenost u jatu i da bi blagovremeno mogle da se sprovedu adekvatne mere koje bi doprinele smanjenju mogucnosti izlucivanja SE preko jaja.