Dengue virus type 3 isolation from Aedes aegypti in the municipality of Nova Iguaçu, State of Rio de Janeiro

In a prospective field study conducted from July 2000 to June 2001, adult Aedes aegypti and Ae. albopictus mosquitoes were caught from the municipality of Nova Iguaçu, State of Rio de Janeiro, Brazil. Virus isolation in Ae. albopictus clone C6/36 cell line and a semi-nested reverse transcription-pol...

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Veröffentlicht in:	Memórias do Instituto Oswaldo Cruz 2002-09, Vol.97 (6), p.799-800
Hauptverfasser:	Lourenco-de-Oliveira, Ricardo, Honorio, Nildimar A, Castro, Márcia G, Schatzmayr, Hermann G, Miagostovich, Marize P, Alves, João C R, Silva, Wellington C, Leite, Paulo J, Nogueira, Rita M R
Format:	Artikel
Sprache:	eng
Schlagworte:	Aedes - virology Aedes aegypti Aedes albopictus Animals Brazil dengue Dengue Virus - genetics Dengue Virus - isolation & purification Female Insect Vectors - virology Male polymerase chain reaction Prospective Studies Reverse Transcriptase Polymerase Chain Reaction reverse transcription Rio de Janeiro Species Specificity virus isolation
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Zusammenfassung:	In a prospective field study conducted from July 2000 to June 2001, adult Aedes aegypti and Ae. albopictus mosquitoes were caught from the municipality of Nova Iguaçu, State of Rio de Janeiro, Brazil. Virus isolation in Ae. albopictus clone C6/36 cell line and a semi-nested reverse transcription-polymerase chain reaction detected only dengue virus type 3 in three pools of Ae. aegypti, despite the co-circulation of DEN-1, DEN-2 and DEN-3 serotypes in that area. No viruses were detected in Ae. albopictus mosquitoes. This virological surveillance consists in a sentinel system alerting for dengue outbreaks.
ISSN:	0074-0276 0074-0276 1678-8060
DOI:	10.1590/S0074-02762002000600009