Hydrogen Peroxide Improves the Efficiency of a Peripheral Blood PCR Assay for Diagnosis of Human Brucellosis

The aim of this study was to verify the usefulness of hydrogen peroxide (H sub(2)O sub(2)) in the elimination of the most common blood contaminants, thus enabling a reduction in the number of washings required for a peripheral blood PCR assay used for the diagnosis of human brucellosis.

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Bibliographische Detailangaben
Veröffentlicht in:	BioTechniques 1999-08, Vol.27 (2), p.248-252
Hauptverfasser:	Queipo-Ortuño, M.I, Garcia-Ordoñez, M.A, Colmenero, J.D, Morata, P
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Brucella - genetics Brucella - isolation & purification Brucellosis - blood Brucellosis - diagnosis Brucellosis - microbiology DNA, Bacterial - isolation & purification Fundamental and applied biological sciences. Psychology Health. Pharmaceutical industry Humans Hydrogen Peroxide Immunological technics applied to diagnosis Industrial applications and implications. Economical aspects Leukocytes, Mononuclear - microbiology Polymerase Chain Reaction - methods
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Beschreibung
Zusammenfassung:	The aim of this study was to verify the usefulness of hydrogen peroxide (H sub(2)O sub(2)) in the elimination of the most common blood contaminants, thus enabling a reduction in the number of washings required for a peripheral blood PCR assay used for the diagnosis of human brucellosis.
ISSN:	0736-6205 1940-9818
DOI:	10.2144/99272bm06